optical fluorescence scanner s chip scanner Search Results


90
CapitalBio Corporation microarray chip scanner boao crystal core luxscan 10 k
Microarray Chip Scanner Boao Crystal Core Luxscan 10 K, supplied by CapitalBio Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Clondiag GmbH atr03 at dna microarray transmission scanner
Atr03 At Dna Microarray Transmission Scanner, supplied by Clondiag GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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atr03 at dna microarray transmission scanner - by Bioz Stars, 2026-10
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86
Hewlett-Packard argon ion laser confocal scanner
Argon Ion Laser Confocal Scanner, supplied by Hewlett-Packard, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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argon ion laser confocal scanner - by Bioz Stars, 2026-10
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90
CapitalBio Corporation luxscantm 10k-b microarray chip scanner
Luxscantm 10k B Microarray Chip Scanner, supplied by CapitalBio Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/optical+fluorescence+scanner+s++chip+scanner/luxscan+10+k+microarray+scanner/pmc06746534-186-33-38
Average 90 stars, based on 1 article reviews
luxscantm 10k-b microarray chip scanner - by Bioz Stars, 2026-10
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90
CapitalBio Corporation luxscan tm scanner
Luxscan Tm Scanner, supplied by CapitalBio Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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luxscan tm scanner - by Bioz Stars, 2026-10
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90
CapitalBio Corporation chip scanner
Chip Scanner, supplied by CapitalBio Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/optical+fluorescence+scanner+s++chip+scanner/chip+scanner/pmc05836372-59-53-55
Average 90 stars, based on 1 article reviews
chip scanner - by Bioz Stars, 2026-10
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Lumonics Inc dna chip scanner scanarray lite
Dna Chip Scanner Scanarray Lite, supplied by Lumonics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/optical+fluorescence+scanner+s++chip+scanner/dna+chip+scanner+scanarray+lite/pmc05854903-49-7-13
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95
Sartorius AG surescan dna microarray scanner
. The 3’ and the 5’ target recognition arms bind to the multiplex PCR products of the β-lactamase genes. During the binding process, the padlock probes are circularized and subsequently ligated. The maximum distance of the padlock probe binding region to the 3’ or 5’-ends of the PCR product should not exceed 200 base pairs. Since the padlock probes gets concatenated with the PCR product upon the ligation reaction, longer distances cause an inhibition of subsequent RCAs. The C2CA sequence is needed for the circle-to-circle amplification and comprises an AluI restriction site for monomerization of the amplification products. The barcode sequence is derived from the 16S rRNA gene. This allowed us to halve the number of <t>microarray</t> probes because the C2CA products and 16S rRNA gene PCR products are detected on the same microarray probe but in different fluorescence channels.
Surescan Dna Microarray Scanner, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
surescan dna microarray scanner - by Bioz Stars, 2026-10
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99
Thermo Fisher mgu74av2 dna chip scanning
. The 3’ and the 5’ target recognition arms bind to the multiplex PCR products of the β-lactamase genes. During the binding process, the padlock probes are circularized and subsequently ligated. The maximum distance of the padlock probe binding region to the 3’ or 5’-ends of the PCR product should not exceed 200 base pairs. Since the padlock probes gets concatenated with the PCR product upon the ligation reaction, longer distances cause an inhibition of subsequent RCAs. The C2CA sequence is needed for the circle-to-circle amplification and comprises an AluI restriction site for monomerization of the amplification products. The barcode sequence is derived from the 16S rRNA gene. This allowed us to halve the number of <t>microarray</t> probes because the C2CA products and 16S rRNA gene PCR products are detected on the same microarray probe but in different fluorescence channels.
Mgu74av2 Dna Chip Scanning, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/optical+fluorescence+scanner+s++chip+scanner/DNA/pm16251409-89-5-12
Average 99 stars, based on 1 article reviews
mgu74av2 dna chip scanning - by Bioz Stars, 2026-10
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90
Genomic Solutions Inc dna microarray scanner genetac uc4×4
. The 3’ and the 5’ target recognition arms bind to the multiplex PCR products of the β-lactamase genes. During the binding process, the padlock probes are circularized and subsequently ligated. The maximum distance of the padlock probe binding region to the 3’ or 5’-ends of the PCR product should not exceed 200 base pairs. Since the padlock probes gets concatenated with the PCR product upon the ligation reaction, longer distances cause an inhibition of subsequent RCAs. The C2CA sequence is needed for the circle-to-circle amplification and comprises an AluI restriction site for monomerization of the amplification products. The barcode sequence is derived from the 16S rRNA gene. This allowed us to halve the number of <t>microarray</t> probes because the C2CA products and 16S rRNA gene PCR products are detected on the same microarray probe but in different fluorescence channels.
Dna Microarray Scanner Genetac Uc4×4, supplied by Genomic Solutions Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/optical+fluorescence+scanner+s++chip+scanner/dna+microarray+scanner+genetac+uc4%C3%974/us07747390-2461-33-38
Average 90 stars, based on 1 article reviews
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90
KangChen Inc genepix 4000b chip scanner
. The 3’ and the 5’ target recognition arms bind to the multiplex PCR products of the β-lactamase genes. During the binding process, the padlock probes are circularized and subsequently ligated. The maximum distance of the padlock probe binding region to the 3’ or 5’-ends of the PCR product should not exceed 200 base pairs. Since the padlock probes gets concatenated with the PCR product upon the ligation reaction, longer distances cause an inhibition of subsequent RCAs. The C2CA sequence is needed for the circle-to-circle amplification and comprises an AluI restriction site for monomerization of the amplification products. The barcode sequence is derived from the 16S rRNA gene. This allowed us to halve the number of <t>microarray</t> probes because the C2CA products and 16S rRNA gene PCR products are detected on the same microarray probe but in different fluorescence channels.
Genepix 4000b Chip Scanner, supplied by KangChen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/optical+fluorescence+scanner+s++chip+scanner/genepix+4000b+chip+scanner/pmc05340452-45-5-9
Average 90 stars, based on 1 article reviews
genepix 4000b chip scanner - by Bioz Stars, 2026-10
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90
Chipron GmbH chip scanner pf 2700
. The 3’ and the 5’ target recognition arms bind to the multiplex PCR products of the β-lactamase genes. During the binding process, the padlock probes are circularized and subsequently ligated. The maximum distance of the padlock probe binding region to the 3’ or 5’-ends of the PCR product should not exceed 200 base pairs. Since the padlock probes gets concatenated with the PCR product upon the ligation reaction, longer distances cause an inhibition of subsequent RCAs. The C2CA sequence is needed for the circle-to-circle amplification and comprises an AluI restriction site for monomerization of the amplification products. The barcode sequence is derived from the 16S rRNA gene. This allowed us to halve the number of <t>microarray</t> probes because the C2CA products and 16S rRNA gene PCR products are detected on the same microarray probe but in different fluorescence channels.
Chip Scanner Pf 2700, supplied by Chipron GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/optical+fluorescence+scanner+s++chip+scanner/chip+scanner+pf+2700/pmc09274830-48-6-21
Average 90 stars, based on 1 article reviews
chip scanner pf 2700 - by Bioz Stars, 2026-10
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Image Search Results


. The 3’ and the 5’ target recognition arms bind to the multiplex PCR products of the β-lactamase genes. During the binding process, the padlock probes are circularized and subsequently ligated. The maximum distance of the padlock probe binding region to the 3’ or 5’-ends of the PCR product should not exceed 200 base pairs. Since the padlock probes gets concatenated with the PCR product upon the ligation reaction, longer distances cause an inhibition of subsequent RCAs. The C2CA sequence is needed for the circle-to-circle amplification and comprises an AluI restriction site for monomerization of the amplification products. The barcode sequence is derived from the 16S rRNA gene. This allowed us to halve the number of microarray probes because the C2CA products and 16S rRNA gene PCR products are detected on the same microarray probe but in different fluorescence channels.

Journal: Bio-protocol

Article Title: Detection of Pathogens and Ampicillin-resistance Genes Using Multiplex Padlock Probes

doi: 10.21769/BioProtoc.2504

Figure Lengend Snippet: . The 3’ and the 5’ target recognition arms bind to the multiplex PCR products of the β-lactamase genes. During the binding process, the padlock probes are circularized and subsequently ligated. The maximum distance of the padlock probe binding region to the 3’ or 5’-ends of the PCR product should not exceed 200 base pairs. Since the padlock probes gets concatenated with the PCR product upon the ligation reaction, longer distances cause an inhibition of subsequent RCAs. The C2CA sequence is needed for the circle-to-circle amplification and comprises an AluI restriction site for monomerization of the amplification products. The barcode sequence is derived from the 16S rRNA gene. This allowed us to halve the number of microarray probes because the C2CA products and 16S rRNA gene PCR products are detected on the same microarray probe but in different fluorescence channels.

Article Snippet: Pipettes ( e.g ., Sartorius, catalog numbers: 728020, 728050, 728060 and 728070) Microbiological incubator shaker ( e.g ., IKA, model: KS 4000 i control) Tabletop centrifuge for 1.5 ml tubes ( e.g ., Eppendorf, model: 5424) Roche MagNA Lyser Instrument (Basel, Switzerland) Thermomixer comfort (Eppendorf, Hamburg, Germany) Epoch Microplate spectrophotometer (Biotek, Winooski, VT, USA) Biosan DNA/RNA UV-cleaner box (Warren, MI, USA) (recommended, see Note 4) Thermal cycler ( e.g ., Thermo Fisher Scientific, Applied Biosystems, model: GeneAmp TM PCR System 2700) (Paisley, UK) Heraeus Megafuge 40R with a TX-750 rotor (Thermo Fisher Scientific, Thermo Scientific TM , model: Heraeus TM Megafuge TM 40R, catalog number: 75004518; TX-750 rotor: Thermo Fisher Scientific, Thermo Scientific TM , catalog number: 75003180) and inserts for plates (Thermo Fisher Scientific, Thermo Scientific TM , catalog number: 75003617) and Falcon tubes (Thermo Fisher Scientific, Thermo Scientific TM , catalog number: 75003608) Slide humidity incubation box ( e.g ., LabScientific, catalog number: HIC-3) GeneMachines Omnigrid 100 contact arrayer (Madison, WI, USA) International Microarray Pin Stealth 3 SMP3 (Telechem, catalog number: SMP3) Agilent SureScan DNA Microarray Scanner (Santa Clara, CA, USA) Autoclave Sartorius arium pro UV ultrapure water system (Sartorius, model: arium ® pro)

Techniques: Multiplex Assay, Binding Assay, Ligation, Inhibition, Sequencing, Amplification, Derivative Assay, Microarray, Fluorescence

For the species identification, the 16S rRNA gene is amplified using universal 16S primers (A1). Subsequently, the PCR products are labeled using a linear PCR and Cy5-modified cytosines (A2). In parallel, β-lactamase genes are pre-amplified in a 33-plex PCR (B1). Then, the padlock probes are hybridized (B2) and ligated (B3) using the β-lactamase PCR products as template and amplified in a first rolling circle amplification (RCA) (B4). The RCA products comprise a C2CA sequence and corresponding 16S rRNA barcode sequences. In a next reaction, the RCA products are monomerized (B5) using a restriction enzyme and circularized (B6) to serve as a new template for a second RCA (B7). These C2CA products are labelled in a linear PCR reaction using Atto532-modified cytosines (B8). Finally, the labelled amplification products from the 16S rRNA gene PCR and from the multiplex padlock assay are pooled together and hybridized to a microarray detecting the products at two different fluorescence wavelengths (C).

Journal: Bio-protocol

Article Title: Detection of Pathogens and Ampicillin-resistance Genes Using Multiplex Padlock Probes

doi: 10.21769/BioProtoc.2504

Figure Lengend Snippet: For the species identification, the 16S rRNA gene is amplified using universal 16S primers (A1). Subsequently, the PCR products are labeled using a linear PCR and Cy5-modified cytosines (A2). In parallel, β-lactamase genes are pre-amplified in a 33-plex PCR (B1). Then, the padlock probes are hybridized (B2) and ligated (B3) using the β-lactamase PCR products as template and amplified in a first rolling circle amplification (RCA) (B4). The RCA products comprise a C2CA sequence and corresponding 16S rRNA barcode sequences. In a next reaction, the RCA products are monomerized (B5) using a restriction enzyme and circularized (B6) to serve as a new template for a second RCA (B7). These C2CA products are labelled in a linear PCR reaction using Atto532-modified cytosines (B8). Finally, the labelled amplification products from the 16S rRNA gene PCR and from the multiplex padlock assay are pooled together and hybridized to a microarray detecting the products at two different fluorescence wavelengths (C).

Article Snippet: Pipettes ( e.g ., Sartorius, catalog numbers: 728020, 728050, 728060 and 728070) Microbiological incubator shaker ( e.g ., IKA, model: KS 4000 i control) Tabletop centrifuge for 1.5 ml tubes ( e.g ., Eppendorf, model: 5424) Roche MagNA Lyser Instrument (Basel, Switzerland) Thermomixer comfort (Eppendorf, Hamburg, Germany) Epoch Microplate spectrophotometer (Biotek, Winooski, VT, USA) Biosan DNA/RNA UV-cleaner box (Warren, MI, USA) (recommended, see Note 4) Thermal cycler ( e.g ., Thermo Fisher Scientific, Applied Biosystems, model: GeneAmp TM PCR System 2700) (Paisley, UK) Heraeus Megafuge 40R with a TX-750 rotor (Thermo Fisher Scientific, Thermo Scientific TM , model: Heraeus TM Megafuge TM 40R, catalog number: 75004518; TX-750 rotor: Thermo Fisher Scientific, Thermo Scientific TM , catalog number: 75003180) and inserts for plates (Thermo Fisher Scientific, Thermo Scientific TM , catalog number: 75003617) and Falcon tubes (Thermo Fisher Scientific, Thermo Scientific TM , catalog number: 75003608) Slide humidity incubation box ( e.g ., LabScientific, catalog number: HIC-3) GeneMachines Omnigrid 100 contact arrayer (Madison, WI, USA) International Microarray Pin Stealth 3 SMP3 (Telechem, catalog number: SMP3) Agilent SureScan DNA Microarray Scanner (Santa Clara, CA, USA) Autoclave Sartorius arium pro UV ultrapure water system (Sartorius, model: arium ® pro)

Techniques: Amplification, Labeling, Modification, Sequencing, Multiplex Assay, Microarray, Fluorescence